B-rafOncogene: Activation by Rearrangements and Assignment to Human Chromosome 7

Assignment of the T-antigen gene of simian virus 40 to human chromosome C-7.

Hybrid cell clones between mouse cells deficient in thymidine kinase (EC 2.7.1.21) and two different human cell lines transformed by simian virus 40 (SV40) and deficient in hypoxanthine phosphoribosyltransferase (EC 2.4.2.8) were examined for SV40 tumor (T) antigen(s). Concordant segregation of the gene(s) for SV40 T antigen and human chromosome C-7 was observed in these hybrids. The human chro...

How to recognize and diagnose chromosome rearrangements

In most eukaryotes, chromosome rearrangements are recognized cytologically by visibly altered chromosome structure, or genetically by altered linkage relations. The first translocations in Neurospora were detected and verified using these methods (McClintock 1945, Houlahan et al. 1949). However, characteristics of Neurospora provide an easier method that depends on ascospore pigmentation, and t...

Human insulin receptor gene. Data supporting assignment to chromosome 19.

Somatic cell hybrid clones constructed by crossing human skin fibroblasts with mouse L cells have been examined for expression of human insulin receptors, using a monoclonal antibody directed against the human insulin receptor. Data obtained in this study support the assignment of the human gene for the insulin receptor to chromosome 19.

Assignment of LCN1 to human chromosome 9 is confirmed.

Regional Assignment of Ptpre Encoding Protein Tyrosine Phosphataes ε to Mouse Chromosome 7F3

Protein tyrosine phosphatases (PTPases) regulate the tyrosine phosphorylation of target proteins in‌volved in several biological activities including cell proliferation and transformation. Protein tyrosine phosphatase E (PTPE) contains duplicated PTPase-like domains and a short extracellular region. Us‌ing the fluorescence in situ hybridization method, the gene encoding PTPE (locus symbol Ptpre...